DNA
Part:BBa_K2100029:Design
Designed by: Kathleen Brandes Group: iGEM16_MIT (2016-10-17)
pEXPR pERE6:eYFP
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6
Illegal EcoRI site found at 18
Illegal EcoRI site found at 333
Illegal EcoRI site found at 343
Illegal XbaI site found at 42
Illegal PstI site found at 581 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6
Illegal EcoRI site found at 18
Illegal EcoRI site found at 333
Illegal EcoRI site found at 343
Illegal PstI site found at 581 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6
Illegal EcoRI site found at 18
Illegal EcoRI site found at 333
Illegal EcoRI site found at 343 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6
Illegal EcoRI site found at 18
Illegal EcoRI site found at 333
Illegal EcoRI site found at 343
Illegal XbaI site found at 42
Illegal PstI site found at 581 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6
Illegal EcoRI site found at 18
Illegal EcoRI site found at 333
Illegal EcoRI site found at 343
Illegal XbaI site found at 42
Illegal PstI site found at 581
Illegal AgeI site found at 364 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This composite part expression vector was created by an LR reaction via gateway cloning. It is a promoter (flanked by L4, R1 sites) and a gene (flanked by L1, L2 sites) cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.
Source
This is from a synthetic promoter and a gene from a jellyfish's genome.
References
Sathya G, Li W, Klinge CM, Anolik JH, Hilf R, Bambara RA. Effects of multiple estrogen responsive elements, their spacing, and location on estrogen response of reporter genes. Mol Endocrinol. 1997;11(13):1994–2003.